Sequence of the glyceraldehyde-3-phosphate dehydrogenase gene fromBacillus subtilis

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Evolution: The Case of the Glyceraldehyde-3-Phosphate Dehydrogenase Gene

The enzyme Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) catalyses the decomposition of glucose. The gene that produces the GAPDH is therefore present in a wide class of organisms. We show that for this gene the average value of the fluctuations in nucleotide distribution in the codons, normalized to strand bias, provides a reasonable measure of how the gene has evolved in time.

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Glyceraldehyde-3-phosphate dehydrogenase (GAPDH, EC 1.2.1.12) mRNA level quantitation is widely used as an invariant control for gene expression analysis. Only three vertebrate GAPDH cDNAs have been hitherto isolated: human (1), rat (2) and chick (3). In order to get a probe for RNAse-mapping experiments on hamster {Cricetulus griseus) mRNAs, we constructed a cDNA library in pBluescript KSH (St...

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Differential expression and sequence analysis of the maize glyceraldehyde-3-phosphate dehydrogenase gene family.

Two cDNA clones for maize cytosolic glyceraldehyde-3-phosphate dehydrogenase are described. One is about 97% similar in coding capacity to a previously published clone [Brinkmann et al. (1987). J. Mol. Evol. 26, 320-328], while the other shows only 88% similarity. Evidence points toward the three cDNAs being the products of three genes, to be called Gpc1, Gpc2, and Gpc3. When the least similar ...

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Nucleotide sequence of the glyceraldehyde-3-phosphate dehydrogenase gene from Cryphonectria parasitica.

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It has been suggested that the energy required for sperm motility is produced by oxidative phosphorylation while glycolysis seems to be an important source for ATP transmission along the flagellum. Some studies have investigated the chemical and kinetic properties of the enzyme glyceraldehyde 3-phosphate dehydrogenase to identify any changes in the regulation of glycolysis and sperm motility. I...

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ژورنال

عنوان ژورنال: Nucleic Acids Research

سال: 1989

ISSN: 0305-1048,1362-4962

DOI: 10.1093/nar/17.3.1251